Improved techniques for automatic updating of playlists for a media system that stores and plays media content for a user of the media system. Between the transmission input shaft and a transmission output shaft gear elements are disposed in a housing for the purpose of implementing different gear ratios. The simulation controller is comprised of a timing wheel, an event-processor, a threadprocess dispatch engine, a token processor, and a resource-allocator. Therefore, an unnecessary perpendicular magnetic field is not easily generated by the magnetic flux. Also provided are methods for detecting the presence of and isolating hematopoietic stem cells in a heterogeneous cell suspension and for assessing gene expression in a tissue sample. A user may orient and insert a needle into the entry site according to the direction of the pointer, by just viewing the pointer, without the needle being constrained by the pointer.