A calibration engine to eliminate disconnects between both manual and automated calibration procedures. The invention also relates to a computer arrangement including a processor and such a computer-readable medium, as well as to a method of updating sets of data having such tagged-data structures. The optical lens includes an inner curved surface and an outer curved surface. Thereafter, a silicon oxide layer is formed over the substrate to cover the one-time programmable read only memory cell, the logic device and the exposed surface of the substrate. The present also discloses the use of TSG101 as a prognostic, diagnostic marker and a potential therapeutic target in cancer, especially ovarian cancer.