The phacoemulsification needle according to the invention comprises a shaft, a tip disposed at a distal end of said shaft, the tip having a larger outer diameter than said shaft and an aspiration lumen extending through said shaft and said tip. In the surface inspection method of the present invention, a resistive film having an arbitrarily determined thickness t1 is first coated on a surface of a sample. The fluid filtration devices, which can be configured to filter a wide variety of fluids, comprises: an influent feed tube; an influent receiving bowl in fluid communication with the influent feed tube; and a plurality of radial arms having filters therein configured to rotate about an axis within the influent receiving bowl. Layered views of the recovered architectures are generated. The best mode pump or pumps used is one or more vacuum pumps.