An instrument having an illumination source configured to illuminate a field of illumination on a surface of a substrate that is configured to hold a sample. Specifically these compounds may be selected from the group consisting of tetrafluoroethane, hexafluoropropane, pentafluoropropane, tetrafluoropropane, trifluoropropane, difluoropropane, octafluorobutane, hexafluorobutane, pentafluorobutane, nonafluorobutane, difluorobutane, trifluoro-2-methoxyethane and bissulfide. The link may establish a relationship a source resource has to a target resource. In addition, embodiments of the present invention provide an assignment mechanism to maximize utilization of the multiple transmit queues by queuing packets corresponding to each transmit request in a next available transmit queue defined for a given priority level.