Methods are provided for isolating and using a whole-saliva leech extract. The wire is mounted in the housing to vibrate at a natural frequency. The apparatus greatly improves synthetic filter attachment to a filter tower frame in an ink cartridge. At least one gap is defined between the first face and at least one of the first and second side plates. The apparatus uses ultrasonic wave to distinguish running direction and speed of vehicles, and can send caution speech information with accuracy to a vehicle whose speed must be limited.