A device for treating a substance containing at least one undesirable organism, using a pulsed electrical field. A map comprising a plurality of map layers may be obtained, where each map layer associates locations in the BSA with spatially variable Forward Link Calibration values for the antenna derived from the updated BSA data, wherein each spatially variable FLC value is associated with a corresponding location in the updated BSA data. This is achieved by determining circularity errors of machine elements which have an influence on the register, assigned to their angular positions and, from these, the correction values for the control of the production of the color separations on the at least one image cylinder being determined. The second device is mounted P-side up at a spaced apart location on the substrate. The present invention also relates to a method of eradicating insects, particularly those found in earthen colonies, by treating the insects with a non-lethal knockout agent, such as trans-dichloroethylene, in combination with a suitable insecticide for the targeted insects. Also disclosed is the supply of power to said first and second amplifiers from a floating power supply means coupled to either an or the output of the output stage so that the voltage of the floating power supply will follow substantially an output voltage of the output stage.