When a low voltage event occurs in a utility grid, a load on a spindle system is repressed so that a damage is prevented. The device includes an infrared transmissive layered window assembly, a means for inducing a thermal gradient in sample tissues. The recognition module determines the position of subsequently detected locations relative to line segments connecting previously detected locations. By providing and using RLF from both local and remote endpoints, the anticipated delay\u2014for packets that have not yet arrived\u2014may be used to preemptively adjust the buffer, thereby minimizing packet loss without introducing unnecessary delay. The method yields a notably high number of CD34+, ALDHbr andor ABCG2-expressing cells, comprising hematopoietic stem cells, permitting the use of the cells with no or minimal expansion.