A storage device failure in a computer storage system can be analyzed by the storage system by examining relevant information about the storage device and its environment. An object positioning and data integrating system comprises EO sensors, a MEMS IMU, a GPS receiver, a laser ranger, a preprocessing module, a segmentation module, a detection module, a recognition module, a 3D positioning module, and a tracking module, in which autonomous, reliable and real time object positioning and tracking can be achieved. The cassette parts are preferably made of PETG and have bonded thereto rupturable membranes preferably made of PET. An embodiment includes forming a sourcedrain region on a substrate, patterning a portion of the sourcedrain region to form segments, etching the segments to substantially separate an upper section of each segment from a lower section of each segment, and filling the space between the segments with an insulating material. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. Arranged inside the cavity are ejectors that are supplied via suitable means with a motive-fluid flow which in turn excites the flow, preferably a circumferential flow or a helical flow.