Positive-working imageable elements comprise a radiation absorbing compound and inner and outer layers on a substrate having a hydrophilic surface. In this manner, a coupling coefficient \u03ba2 in a front end face region of a conventional semiconductor laser is smaller than a coupling coefficient \u03ba1 in a rear end face region and is larger than 100 cm\u22121. The pin may have a rotationally non-symmetrical shaft portion to provide different wear surfaces that engage a center link of the section of chain. The calibration may be updated after evaluating the calibration set for best calibration based on inclusion criteria with newly received reference analyte data.