The present invention provides isolated bovine Neospora cultures. The method includes: receiving, by a first traffic offload function entity, user information of a user equipment sent by a core network device; obtaining, a traffic offload policy and matching the traffic offload policy with the user information; and offloading traffic corresponding to the user equipment according to the user information that successfully matches the traffic offload policy. A flange surrounding an inlet opening is positioned spaced from the collection area. The application can begin to access the initial portions of the file by accessing the modified file. The method includes the step of: alternately repeating a superposition operation for superposing a pulse current on a lamp current applied to the high pressure discharge lamp for a predetermined time period and a superposition stopping operation for stopping the superposition of the pulse current for a predetermined time period. The threshold level may be established based upon a packet loss rate corresponding to a significant degree of service quality degradation.