Methods for detecting the presence or amount of Johnsongrass or Johnsongrass hybrid genetic material in a biological sample, preferably the detection of the presence or amount of Johnsongrass or Johnsongrass hybrid seed in a seed sample, are disclosed. The first and second access paths have a different access technology. The glycerin produced from plant fats, animal fats or the like or pure product synthesized chemically or a discharge containing glycerin generated at the production of diesel fuel oil from fats, in which the fats are subjected to a transesterification with alcohol in the presence of an alkali catalyst in order to obtain fatty acid ester is preferably used as a starting material. In the halo implantation, the range of projection is set to reach the inside of a buried oxide film. That is, it provides a defecation-promoting agent comprising a compound having an adenosine A2 receptor antagonism, preferably an adenosine A2b receptor antagonism, or a salt thereof. The negative impact card can be used in all poker games and other card games where the player has a limited number of cards from which to determine a win.