A system, including methods and apparatus, for microfluidic analysis of a nucleic acid target in a nucleic acid mixture. An annulus is formed between the rotor and the stator. The cam buckle member may include a hook section and a buckle section, wherein the buckle section may include a central opening and at least one slot. Simultaneously, an assisted ion beam provides ions directly to the substrate, thus improving the softness of the free layer and smoothness of the spacer layer. This process may be repeated for different sizes of the at least one sleep transistor to determine an optimum size of the at least one sleep transistor to optimize at least one parameter of the logic circuit.