A metal chelated dyestuff for inkjet recording is described, which is a water soluble azo metal chelated compound formed from an azo compound represented by the following general formula and a metal element and is good at the clearness of color tone, light resistance, indoor discoloring and fading property, solubility, storage stability, and the like. Other aspects of the invention include methods for rendering gram negative bacterial cells bearing the Hte region, such as E. Because some of the underlying assets may not be traded in a liquid market at the time of valuation of the fund, a reliable estimate of the value of such underlying assets must be made based on available data related through historical correlations. The controller controls the actuators to operate through a plurality of cycles in a substantially unattended fashion.