In the method, a set of limits applicable to a test rod pattern design are defined, and a sequence strategy for positioning one or more subsets of the test rod pattern design is established. A temperature of the die is measured. The frame includes first and second clamp members for securement at spaced positions along the rigid pipe typically attached to the vacuum head in the central vacuuming system. Since the side light-emitting device emits most the light in the lateral direction, using the side light-emitting device as a light source in a backlight unit allows light to be mixed evenly. The device, methods and kits described herein have application for detection and measurement of biomolecular species including polynucleotides, proteins, polysaccharides and the like.