The present invention discloses transforming growth factor alpha HII polypeptides and polynucleotides encoding such polypeptides. It also shows that IPGs are second messengers for allergic stimulation as the addition of some types of purified IPGs to non-allergen stimulated cells resulted in histamine release or degranulation. The inflatable cuff in one embodiment includes an integrated hand pump for convenient inflation and deflation. In the locking position, the free end portion of the plunger projects from the housing to extend into an aperture in the other member, when the housing is secured to the one member, and thereby prevent relative movement between the members. A portion of the substrate of the donor wafer is then cleaved.