Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The fifth wheel hitch may be arranged to be secured to a hitch ball, such as a gooseneck hitch ball. The treatment device also includes an administration mechanism that is coupled to both the extraction mechanism and the lysis mechanism. The signal mixture circuit includes a combination of a capacitor and a transmission switch, stores pixel signals from pixels included in a same-row and same-color pixel group and mixes the signals together. The outer sheaths of the filamentary members are heat fused at least in the regions near the cut edges to keep the cut edges from fraying or the filamentary members from pulling out of the substrate. These cell lines represent unique cancer cell and cancer stem cell models for preclinical prostate cancer studies and CSC-targeted drug development, which is of high value for pharmaceutic companies producing anti-cancer agents, as well as for the broad range of basic and translational research focused on cancer cell and CSC biology, stem cell behavior, cancer development and metastasis.