The present invention provides methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell, as well as, enzymes, polypeptides, and a variety of vector constructs useful therefore. The pin assembly includes a set of pins. The sliders have slider grooves engaging with the bosses. The outer wall also includes a sealing portion to capture a seal assembly between the sealing portion and a surface of a body of the fluid valve. , tables of APIs functional arguments, and each of the potential domain objects include one or more attributes.