A method for the diagnosis of bladder cancer andor for determining a tumor stage of bladder cancer, comprising the step of determining the presence or absence or amplitude of at least six polypeptide markers in a sample, wherein said polypeptide markers are selected from markers 1 to 836, which are characterized by the values for the molecular masses and migration times. Such modifications include generating insertions, deletions, substitutions, andor point mutations at any chosen site in the independent origin based cloning vector. The diamond material layer has a first surface and a substrate. The request is forwarded via a secure tunnel from the on-premise platform to the requested cloud resource.