The isolation and characterization of a metastasis tumor suppressor gene KAI1 is disclosed and diagnostic methods and gene therapy approaches utilizing reagents derived from the nucleotide and deduced amino acid sequences of the KAI1 gene are provided. The process includes methods of separation of the tocopherols from residues of vegetable oil refining and other various food sources by dissolving in organic solvents and eluting over non-ionic adsorbent resins. An outer cam collar and a concentrically enclosed inner ring are mounted upon the cylinder end into which the piston inserts. Suitable blowing agent combinations include: a composition containing carbon dioxide and at least one hydrofluorocarbon having a boiling point of 30 C. Fabrication methods of such non-planar graphite-based devices are also provided. It is an aspect of the present invention to be applicable to a variety of different semiconductor micromechanical devices, for instance actuators, motors, sensors, spatial light modulators, and deformable mirror devices.