A method and vaccine for treatment of pythiosis in humans and animals is described. The clamp circuit includes a buffer unit and a clamp unit. The compressible base-seal and the flange seal form an enclosure seal when the first L-shaped door and the second L-shaped door are closed. The present invention determines the compensation factor based on assessing the output of the camera against a known light source for a specific set of imaging conditions in the imaging system. The drilling fluids do not contain heavy metals and are rheologically tolerant to contaminants such as cement, anhydrite and sodium and temperatures as high as about 350\xb0 F.