Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids using this delta-8 desaturase in plants and oleaginous yeast are disclosed. The outlet chamber member has wings that have free ends. The electrically conductive polyimides of the present invention have an excellent balance of properties relative to conventional polyimides having a conductive filler, due at least in part to the film’s water content, degree of imidization and polymer orientation. The threaded coupler includes a key, or a recess to mate with a key, to substantially prevent rotation of the threaded coupler with respect to the hose coupler. In other words, the relationship between the thickness A of the one sidewall facing toward the uncoated portion of the positive electrode sheet and the thickness B of the other sidewall is A>B.