Provided are hydrolases, including lipases, saturases, palmitases andor stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. The transmission medium is capable of a carrying a plurality of wavelengths organized into bands. The wire electrode includes a material the same as a material constituting the sensing electrode. A final N-symbol decision regarding the current transmitted symbol corresponds to the vector of maximum length, calculated after the -th step of the iterative procedure.