The present subject matter relates to methods and systems for assessing an athlete’s risk of injury and for determining athletic performance training and treatment programs. The particles are inputted into at least one of the plurality of inlets and are deflected through a series of parallel flow streams flowing from the plurality of inlets to the plurality of outlets while being deflected toward the second wall, wherein streams in the parallel flows comprise a reagent. The method may further include forming a barrier layer within the opening of the etch stop layer.