A method and system for auto-focusing is disclosed where the number of the fractal pixels having a fractal dimension within a predetermined range is determined. The mixture then passes through a sorting mechanism that separates the core pieces from the mixture. Methods of using these compounds for modulation of ADAM15 expression and for diagnosis and treatment of disease associated with expression of ADAM15 are provided. The present invention is to obviate the above disadvantages. The second substrate is thinner than the first substrate, and has a central hole whose diameter is larger than that of the first substrate.