The present invention provides for predicting peak cylinder temperatures above which knock in an engine may become more frequent and then provides one or more actuation approaches to reduce the knock of the engine while maintaining engine performance. The recombinant adenovirus of the present invention can be used in the treatment of various cancers including cervical cancer, breast cancerand colon cancer. The surface coating composition can be used to coat at least part of a surface of an assembly for use in hydrocarbon production, transportation, storage or processing.