Text captioned telephony, in which a telephone conversation is accompanied by text captions to aid in hard-of-hearing users, is implemented without the use of specialized text captioned telephone terminals by using a combination of an Internet appliance such as a computer and a telephone or by generalized voice over Internet protocol telephones. The invention provides troponin C, troponin C binding peptide, and troponin I, or active fragments or analogues thereof as convenient tags and affinity ligands for immobilizing, attaching, or purifying proteins or other molecules. Pharmaceutical compositions and methods of treatment are also included. The hybrid adjunct material is releasably retained on the cartridge body and configured to be delivered to tissue by deployment of the staples in the cartridge body.