An ozone generating system includes a water reservoir for accepting water from a water source, such as an inverted water bottle or a point of use water source. The method further includes injecting a second quantity of fuel into the engine cylinder at a second predetermined time after the first predetermined time when the piston is advanced by a predetermined advance value before the piston reaches the top dead center of the compression stroke. A controller variably controls a flow of fuel to the nozzle according to a temperature of water in the tank. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue, or precursor cells of the selected tissue, with multiple doses of at least one inhibitor of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue. Further, the method of the present invention has a simple fabrication process, which can benefit mass production and lower cost.