The present invention is directed to a screed assembly that includes a strike-off. Each gain stage includes a gain transistor and a cascode transistor to form a cascode amplifier, and a current diversion transistor to divert current away from a cascode transistor to reduce gain in the stage. The methods can further include laboratory culturing steps to confirm and possibly expand the determinations made using the in silico methods, and to produce a cultured cell, or population of cells, with optimal functions.