Provided are methods, systems, and computer readable media for user interaction with database methods and systems. In preferred embodiments, the methods of the invention comprise contacting the biological sample with lysis solution, and subjecting the mixture to ultrasound, thereby selectively lysing the endogenous cells in the biological sample. A refractive index of the interface layer is smaller than a refractive index of the protective layer. The Voice Packet Forwarding task builds a transmit payload buffer for voice samples received from the synchronous interface, builds the necessary protocol headers and forwards the packet to the packet oriented interface. Such data includes actual wholesale sold amounts, actual retail sold amounts, actual purchase amounts and actual reconditioning amounts, as well as additional relevant information, such as stock date, sold date, gross profit, geographical data, etc.