Compositions suitable for use as signal generation components of an immunoassay, and methods for their use. The minus ion generator carries a high minus voltage, and the positive electrode carries a high plus voltage. The mapping determination is based at least in part on whether the shared data channel is associated with a legacy wireless technology or an advanced wireless technology. The fluid handling system also has a second fluid handling module separate from the first module which is configured to interface with the main instrument. An analog-to digital converter samples the filtered signal at a sample rate of 2 to generate a corresponding multi-bit digital sample stream.