The present invention relates to a method and device of inserting a coated electric heating wire into a hot water tube and to an apparatus for a sealing plug mounted at an end of said hot water tube. A disclosed method, directly and completely on the microtiter plate, comprises binding DNA to microtiter plate, reacting abasic sites of DNA with a chemical reagent ), and performing a biotin avidin horseradish peroxidase technique to tag ARP with a biotin residue for subsequent colorimetric analysis. An optic device adjacent the down conversion material at least partially surrounds the radiation source.