A method of modulating and in particular, reducing nitrous oxide emission from a substrate for growing on or more plants by applying a microbial based soil additive and a fertilizer blend to the substrate. The clip includes a release edge configured to engage a flared lip of a dovetail shim. Illustrative compositions comprise an adenovirus and an adjuvant such as, for example, monophosphoryl lipid A, an alkyl glucosaminide phosphate, a saponin, or a combination thereof. The tucker assembly further includes a roller mounted by a pin to the tucker element such that the tucker element rolls along a surface defined by the slot. In another embodiment, a compression protocol is provided for compressing trace messages on-chip prior to transmitting the messages to an external system or storing the messages in memory.