Disclosed is a DLL circuit for avoiding stuck and harmonic locking errors by utilizing a frequency divider and an inverter. The connecting rod type two-level locking device includes a transmission shaft, a door opening arm, a first roller, a second roller, a first locating pin, a standstill locking disk and a linkage mechanism. The driver alert apparatus includes a speaker and visual indicator connected to be controlled by the controller, such that the speaker and visual indicator are controllably activated by the controller based on the received data. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. The imaging device which is mounted on or otherwise coupled to the cutting blade is configured to be in an imaging position when the cutting blade substantially closes the aperture or cutter window. The turbine can include an integrated manifold comprising a plenum defined by a manifold disposed in connection with a divider plate and opposite a shielding plate comprising a plurality of apertures.