The present invention relates to gene expression profiles, microarrays comprising nucleic acid sequences representing gene expression profiles, and methods of using expression profiles and microarrays. A layout generator is configured to generate a layout based on the information, the cell library for low-threshold cells, and the cell library for high-threshold cells. Then, the sampling nozzle is inserted into an injection port of the flow cell, and the reagent is injected into the flow cell by driving the microsyringe. A B1616 tile in the middle level of hierarchy is a sixteen by sixteen array of B1 blocks. In some embodiments, a cannula seal can include a base portion that engages with a cannula; and a seal portion integrally formed with the base portion that slidebly engages with an instrument shaft such that an insertion frictional force between the seal portion and the instrument shaft for insertion of the instrument shaft is symmetrical and substantially equal with a retraction frictional force.