A language-learning system and a method thereof are provided. The invention provides for methods of imparting hydrophobic properties to such damaged silica dielectric films present on a substrate. The enzymatic activity of the reverse transcriptase is determined by measuring the DNA product produced when an RNA template and a first complementary DNA primer from a suitable region of the encephalomyocarditis virus genome are incubated with a biological sample containing reverse transcriptase in the presence of the drug to which resistance is being determined. CNV that can be determined according to the present method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions andor duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample.