A method of determining the \u201cphasing\u201d of an internal combustion engine includes two banks of cylinders each supplied by its own intake manifold. The reception apparatus and transmission apparatus can link carrier aggregation technology and scalable bandwidth technology by supporting a scalable bandwidth having different bandwidths in size in a carrier aggregation environment, thereby enhancing compatibility between different wireless communication systems. Said medium further comprises a urea derivative, adapted to interact with said staining reagent and thereby providing an enhanced staining of said nucleic acids.