A cooling assembly includes a chassis, a circuit board secured to the chassis, a cooling layer, a cooler secured to the circuit board, and a housing receiving the cooler and the circuit board and secured to the chassis. Preferably before the IOBW, the unopened defeathered carcass is washed with such microbiocidal composition. The system includes a control system configured to align the first and second detectors with the at least two collimators using the alignment device. As the plunger is moved during delivery, features on the plunger and features connected to a barrel interact to provide for intermittent stops to the delivery.