Systems and methods of photoaltering a region of a material using a pulsed laser beam. The variable gain amplifier employs a wide swing cascode mirror formed by two cascode transistors and two gain transistors. The multi-layer device preferably consists of three conjoined layers: first layer, intermediate layer, and second layer, and at least one piping. In a preferred embodiment is a glioma-specific immunoreceptor comprising the extracellular targetting domain of the IL-13R2-specific IL-13 mutant IL-13 linked to the Fc region of IgG, the transmembrane domain of human CD4, and the human CD3 zeta chain. For example, electron probe beams with spiral phase dislocations may occur. The method includes a sputtering process for coating a substrate with at least one functional layer, the sputtering process being interrupted at least once by the application of an intermediate layer with a thickness of less than 20 nm.