A bus cycle trapping system includes at least one register, a north bridge, a south bridge and a central processing unit. The base holder includes first and second guide rails. A priority is then assigned to each label associated with a button and each icon associated with a button. The common seam can be closed using adhesive, heat bonding or sewing. The cycle is then displayed for diagnostic purposes, triggers acquisition of ultrasound images, used for altering other processes or calculations or used for performing another act. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.