A pneumatic tire with a double layer innerliner structure bonded to an inner face of the tire carcass for reducing noise generated by air vibrations inside of the tire’s air chamber, and a method of making the tire. All illumination is focused onto the back focal plane of the objective, that from the third path being off-axis for out-of-plane Moir\xe9 analysis. The ion-supplying material lies between the inner wall of the tubular member and the second electrodes and includes a polymer gel containing positive ions and negative ions. An outer liquid outlet is disposed in the outer drain body. The physical addresses are output for use in accessing the memory. Therefore signal transduction pathways mediated by these agents provide targets for drug screening assays designed to identify inhibitors of V-H-ATPase activity and AVO formationacidification.