A filter element includes a filter web pleated in an essentially zigzag manner and made, in particular, of a nonwoven material, for insertion into a holder such as a filter frame andor a filter housing, having at least one end-positioned wall of an essentially flat design. The probe andor the stylus moves relative to the Z-ram such that the calibration artifact remains stationary while the Z-ram is positioned in the first calibration position and the second calibration position. The resulting structure is compact and provides good structural support and cooling for the disconnect clutch and motor. The blades have a step near the gauge section to increase the exposure of the blade cutting structure. Adapters intercept the energy flow to the motor of the tool and re-channels the energy flow through a modular control apparatus, which then controls the flow of energy to the motor. Genes of interest for manipulation to create gene position-shifted RSV include any of the NS1, NS2, N, P, M, SH, M2, M2, L, F or G genes or a genome segment that may be part of a gene or extragenic.