The present invention is directed towards a process of preparing a catalyzed carbonaceous material and preventing polymerization of cracked volatile products during pyrolysis or gasification of carbonaceous materials. The present invention also relates to recombinant perforin polypeptides and nucleic acid molecules derived therefrom and uses thereof. Meanwhile, the flexible substrate winds tightly around sidewalls of tapered shape objects and is set up on, yet covers the base surface of tapered shape object which faces the tip of tapered shape object.