An image forming apparatus includes an image forming apparatus body, an image reading apparatus mounted on the upper portion of the apparatus body, and a postprocessing apparatus having a folding unit which folds a sheet. The first unit serves as the driving unit, which is rotatable around a central axis thereof. The calibration module then calibrates the RSSI module according to the output signal in order to calibrate the output level of the demodulator. Unlike traditional bar code which requires an exclusive space, EMBLs are optimally blended with contents and thus have less interference with the original document layout and can be moved closer to a media associated location. Because of binding to a subtype EDG-2 of LPA receptor, an EDG-2 antagonist is useful in treating andor preventing chronic diseases induced and made chronic by tissue cells whose proliferation is accelerated by LPA mediated by EDG-2. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, andd) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.