The present invention provides methods and systems for an automated method of identifying allele values from data files derived from processed fluorophore emissions detected during the observation of fluorophore labeled nucleotide probes used in analyzing polymorphic DNA are provided. The composition is capable of reacting via hydrosilylation reaction to form a reaction product, such as a silane, a gum, a gel, a rubber, or a resin. The preform is formed so as to have a relative density of 70-90% and an oxygen content of 100-500 ppm. In addition, due to the capabilities of the geographic referenced system, in certain embodiments, the movement of emergency service needs can be anticipated and PSAPs may be conferenced together so that they can respond as a single unit to someone who may be traveling. The non-linearity of the power amplifier is estimated in accordance with the signals at the first location using an inverse model. The assembly further comprises a plurality of standoffs positioned in between the first surface and the abutment to provide a gap.