Methods and compositions for regulating immunity are disclosed. In embodiments where multiple power amplifiers are used, a combiner may be provided to combine outputs of the power amplifiers. The spacer layer is disposed between the first sensing layer and second sensing layer, and includes at least one high pressure spacer zone and at least one low pressure spacer zone. Optionally, a thin, flexible substrate material is perforated during the stitching process. The fuse is cut off during a trimming process.