Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides andor fusion proteins. Thermolysis of N-acylureas at above about 120\xb0 C. Coatings are applied on both surfaces of the beads, respectively. Each handle includes a longitudinal hole having two end openings. The wall of the right auricle is then pierced with the distal end of the leading guide wire. In embodiments, the toner particles may have a core-shell configuration, with the gel in the core, the shell, or both.