A threaded connection comprising a first threaded member threadably connected with a second threaded member and an axial loading member. The cylinder portion has a first opening, a second opening, and a cut and can be in contact with an external conductor of a receptacle inserted from the first opening. Support structures are formed that are disposed so as to support electrically conductive interconnects on various levels of the integrated circuit. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved andor partially cleaved e-tag probes.