High quality epitaxial layers of monocrystalline materials can be grown overlying monocrystalline substrates such as large silicon wafers by forming a compliant substrate for growing the monocrystalline layers. Preparation method and use as medications with antimalarial activity. The blade having opposing brackets which support a grab bar and adjustable striker bar, allowing it to be installed on numerous buckets without modification of attachment or bucket allowing it to be removed quickly. The offset is a function of the first parameter and the third parameter. This allows a significant enlargement of the frozen volume of the treated tissue with the same operation temperature and the outer diameter of the cryoprobe. A thermocouple well is mounted in the at least one thermocouple well receiving portion.