One or more impurities may be incorporated within a metal-containing layer of a metal-containing gate electrode to modify the work function of the metal-containing gate electrode of a transistor can affect the threshold voltage of the transistor. A preferred olefin feedstock is for the production of primary alcohol compositions by skeletal isomerization of the olefins followed by hydroformylation. This novel and improved SERS assay can detect minor spectral differences within strains of a single virus type such as respiratory syncytial virus or influenza virus in the presence of biological media.