A system and method for measuring the capture threshold of a bipolar lead in order to determine an appropriate value for the stimulus pulse energy to be used with the lead by a cardiac rhythm management device. The high-carbon steel wire rod of high ductility is a high-carbon steel wire rod fabricated by hot rolling that that has a carbon content of 0. In some implementations, the locations of the wireless signal transmitters are determined based on identification information encoded in the wireless signals received by the mobile device. The methods can include, for example, treating a biomass containing the PHA to form a pyrrolidone. The discoverable attributes lead to an ability to automatically create sub-nodes of nodes for finer-grained control. Methods and compositions for treating disease or condition modulated by P2X7 are also disclosed.